|
|
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/03/2009 |
Data da última atualização: |
08/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
JOSÉ, A. A. F. B. V.; GAMA, M. A. S.; LANNA, D. P. D. |
Afiliação: |
A. A. F. B. V. José, ESALQ/USP; Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; Dante Pazzanese Duarte Lanna, ESALQ/USP. |
Título: |
Effects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 7, n. 2, p. 284-294, 2008. |
DOI: |
https://doi.org/10.4238/vol7-2gmr417 |
Idioma: |
Inglês |
Conteúdo: |
The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue. MenosThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12... Mostrar Tudo |
Palavras-Chave: |
Adipose tissue explants; Bioactive fatty acid. |
Thesaurus Nal: |
conjugated linoleic acid; lipogenesis; swine. |
Categoria do assunto: |
-- |
URL: |
https://www.funpecrp.com.br/gmr/year2008/vol7-2//pdf/gmr417.pdf
|
Marc: |
LEADER 02768naa a2200217 a 4500 001 1596283 005 2024-02-08 008 2008 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4238/vol7-2gmr417$2DOI 100 1 $aJOSÉ, A. A. F. B. V. 245 $aEffects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs.$h[electronic resource] 260 $c2008 520 $aThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue. 650 $aconjugated linoleic acid 650 $alipogenesis 650 $aswine 653 $aAdipose tissue explants 653 $aBioactive fatty acid 700 1 $aGAMA, M. A. S. 700 1 $aLANNA, D. P. D. 773 $tGenetics and Molecular Research$gv. 7, n. 2, p. 284-294, 2008.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 94 | |
7. | | OLIVEIRA, D. E.; MEDEIROS, S. R. de; LANNA, D. P. D. Nitrogen balance and excretion from grazing lactating cows supplemented with conjugated linoleic acid (CLA). In: ADSA-PSA-AMPA-ASAS JOINT ANNUAL MEETING, 2008, Indianapolis. Abstracts. Indianapolis: ADSA: ASAS, 2008. Disponível em:< http://adsa.asas.org/meetings/2008/abstracts/TOC.htm > Acesso em: 26 mar. 2009. Publicado em: Journal of Animal Science, Champaign, v. 86, E-Supplement 2; Journal of Dairy Science, Champaign, v. 91, E-Supplement 1. P. 487 1 CD-ROM. Ruminant Nutrition: proteins and amino acids - Dairy. O485. Trabalho TH253.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
| |
12. | | BERNDT, A.; CRUZ, G. M. da; LANNA, D. P. D.; TULLIO, R. R.; ALLEONI, G. F.; CORDEIRO, C. A. Composição física da 9-11a costelas de tourinhos de diferentes grupos genéticos em confinamento em relação ao status nutricional na fase de pastejo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba, SP. Anais... Piracicaba: SBZ, 2001. p.1302-1303.Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
13. | | BERNDT, A.; CRUZ, G. M. da; LANNA, D. P. D.; TULLIO, R. R.; ALLEONI, G. F.; ALENCAR, M. M. de. Composição química das 9-11AS costelas de tourinhos Nelore e cruzados terminados em confinamento em relação ao status nutricional na fase de pastejo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 43., 2006, João Pessoa. Produção animal em biomas tropicais: anais. João Pessoa: SBZ: UFPB, 2006. 5 f. 1CD ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
14. | | CHAVES, A. S.; NASCIMENTO, M. L. do; TULLIO, R. R.; BERNDT, A.; ALENCAR, M. M. de; LANNA, D. P. D. Desempenho e características de carcaça de bovinos Nelore em função do consumo alimentar residual. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., Brasília, DF. A produção animal no mundo em transformação: Anais. Brasília: SBZ, 2012.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
15. | | CRUZ, G. M. da; TULLIO, R. R.; ALLEONI, G. F.; LANNA, D. P. D.; ALENCAR, M. M. de; BERNDT, A. Desempenho de machos não-castrados de diferentes grupos genéticos em confinamento em relação ao status nutricional na fase de pastejo. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000, Viçosa, MG. Anais... Viçosa : SBZ, 2000.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
18. | | ALBERTINI, T. Z.; MEDEIROS, S. R. de; TORRES JÚNIOR, R. A. de A.; LANNA, D. P. D. Cow live weight is negatively related to feed efficiency of cow/calf pairs from birth to weaning. In: ADSA-PSA-AMPA-ASAS JOINT ANNUAL MEETING, 2008, Indianapolis. Abstracts. Indianapolis: ADSA: ASAS, 2008. Disponível em:< http://adsa.asas.org/meetings/2008/abstracts/TOC.htm > Acesso em: 26 mar. 2009. Publicado em: Journal of Animal Science, Champaign, v. 86, E-Supplement 2; Journal of Dairy Science, Champaign, v. 91, E-Supplement 1. p. 182 1 CD-ROM. Ruminant nutrition: growing youngstock, calves and heifers. O181. Trabalho 215.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
| |
19. | | BERNDT, A.; LANNA, D. P. D.; CRUZ, G. M. da; TULLIO, R. R.; SAKAMOTO, L. S.; ALENCAR, M. M. de. Prediction of the chemical body composition of Nellore and crossbreed bulls. Journal of Animal Science, v. 95, n. 9, p. 3932-3939, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
Registros recuperados : 94 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|